Steps

Volume (mL)

Total Activity (Units)

Total Protein (mg)

Specific Activity (U/mg)

Fold Purification

% Age Recovery

Crude

176

227.4976

2200

0.1034

-

-

Ammonium Sulphate (40% - 70%)

25

219.0

510

0.4294

4.15

96.27

PEG-4000 (30%)

10

173.60

310

0.56

5.42

76.31

CM-Cellulose Column (Unbound Sample)

2.9

96.96

55.1

1.76

17.02

42.62

DEAE-Cellulose Column

1.8

48.61

3.8

12.79

123.69

21.37