Flow (mL/min) | Eluent A (%) | Eluent B (%) | Duration (min) |
0.6 | 83 | 17 | 0 |
0.6 | 91 | 9 | 20 |
0.6 | 91 | 9 | 30 |
0.6 | 100 | 0 | 35 |
0.6 | 100 | 0 | 50 |
0.6 | 83 | 17 | 51 |
0.6 | 83 | 17 | 55 |