Primer

Nucleotide sequence (5¢®3¢)

Thermocycling conditions

matK_XF1

matK_MALP_R1

TAATTTACGATCAATTCATTC

ACAAGAAAGTCGAAGTAT

94˚C for 4 min, 40 cycles of 94˚C for 50 s, 52˚C for 1 min, and 72˚C for 1 min, 72˚C for 10 min

rbcLa F

rbcLa R

ATGTCACCACAAACAGAGACTAAAGC

CTTCTGCTACAAATAAGAATCGATCTC

94˚C for 1 min, 30 cycles of 94˚C for 45 s, 51˚C for 45 s, and 72˚C for 5 s,

72˚C for 5 min

rpoB F

rpoB R

ATGCAACGTCAAGCAGTTCC

GATCCCAGCATCACAATTCC

94˚C for 4 min, 40 cycles of 94˚C for 30 s, 53˚C for 40 s, and 72˚C for 40 s, 77˚C for 7 min

rpoC1-1F

rpoC1-3R

GTGGATACACTTCTTGATAATGG

TGAGAAAACATAAGTAAACGGGC

94˚C for 4 min, 40 cycles of 94˚C for 30 s, 53˚C for 40 s, and 72˚C for 40 s, 72˚C for 7 min

atpF-atpH F

atpF-atpH R

AACTCGCACACACTCCCTTT

GGRGTTGGTCAAGGTACTGC

94˚C for 3 min, 35 cycles of 94˚C for 30 s, 51˚C for 40 s, and 72˚C for 40 s,

72˚C for 10 min

psbK-psbI F

psbK-psbI R

TTAGCATTTGTTTGGCAAG

AAAGTTTGAGAGTAAGCAT

95˚C for 2 min 30 s, 35 cycles of

95˚C for 30 s, 51˚C for 1 min, and 72˚C for 1 min, 72˚C for 10 min