PCR parameter

Tested range

Optimum concentrations in 25 µl

Template DNA concentration (ng)

10, 20, 30, 40, 50, 60

30

Each Deoxy nucleotide triphosphate (dNTPs) (µM)

100, 150, 200, 250, 300

200

Magnesium Chloride (MgCl₂) mM

0.0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5

1.5

Taq DNA polymerase (U)

0.5, 1.0, 1.5, 2.0, 2.5

2.0

Agarose (%)

1.5, 2.0, 2.5, 3.0

2.0