Primer code | Primer sequence | Annealing temperature (˚C) |
GW1CF | 5’-CAACCACGGAAAAGCGGTTTGTAATGCATG-3’ | 74 |
GW1CFN | 5’-TCTGTGGGGACATCTGTTTCAGGTTG-3’ | 76 |
GW2CF | 5’-TGTGGGGACATCTGTTTCAGGTTG-3’ | 70 |
GW2CFN | 5’-AATACATACCGAAAACGACCCGTC-3’ | 68 |
GW3CF | 5’-AAGGCTGGAGGAATTGCCCTCAAAT-3’ | 72 |
GW3CFN | 5’-ACTGACCAAATTGCGTTGCCTAGAA-3’ | 70 |
GW4CF | 5’-TCTTTTCCACGTCTTCAACATCTGTCT-3’ | 74 |
GW4CFN | 5’-TATGGATCACTGTCCCGAGCTGAAAG-3’ | 76 |
GW5CF | 5’-TAAAACATGCGTCGCTTTCAAGTT-3’ | 64 |
GW5CFN | 5’-TTAAGAGAGTCATTGGATGCAAAC-3’ | 64 |
CYRT-Fd | 5’-TAGCAAAGCATGGGTCTGTG-3’ | 55 |
CYRT-Rv | 5’-GAGTTTGTACAGCTTCCCATTCGTCTC-3’ | 54 |
GW4CR | 5’-GAGTTCAAGCGATGATGTGTCCATGT-3’ | 71 |
CYR1-F-Bam-Tn | 5’-TTTTTTTGGATCCATGGCAGCAGAGCTTGTC-3’ | 78 |
CYR1-R-Kpn-Tn | 5’-TTTTTTTGGTACCCATTGAAAAATTGATTATTA GGTAA-3’ | 69 |
S5RcCyR3 | 5’-TGAACTGCTTCTGTTCTGCAT-3’ | 55 |