Primer code

Primer sequence

Annealing temperature (˚C)

GW1CF

5’-CAACCACGGAAAAGCGGTTTGTAATGCATG-3’

74

GW1CFN

5’-TCTGTGGGGACATCTGTTTCAGGTTG-3’

76

GW2CF

5’-TGTGGGGACATCTGTTTCAGGTTG-3’

70

GW2CFN

5’-AATACATACCGAAAACGACCCGTC-3’

68

GW3CF

5’-AAGGCTGGAGGAATTGCCCTCAAAT-3’

72

GW3CFN

5’-ACTGACCAAATTGCGTTGCCTAGAA-3’

70

GW4CF

5’-TCTTTTCCACGTCTTCAACATCTGTCT-3’

74

GW4CFN

5’-TATGGATCACTGTCCCGAGCTGAAAG-3’

76

GW5CF

5’-TAAAACATGCGTCGCTTTCAAGTT-3’

64

GW5CFN

5’-TTAAGAGAGTCATTGGATGCAAAC-3’

64

CYRT-Fd

5’-TAGCAAAGCATGGGTCTGTG-3’

55

CYRT-Rv

5’-GAGTTTGTACAGCTTCCCATTCGTCTC-3’

54

GW4CR

5’-GAGTTCAAGCGATGATGTGTCCATGT-3’

71

CYR1-F-Bam-Tn

5’-TTTTTTTGGATCCATGGCAGCAGAGCTTGTC-3’

78

CYR1-R-Kpn-Tn

5’-TTTTTTTGGTACCCATTGAAAAATTGATTATTA GGTAA-3’

69

S5RcCyR3

5’-TGAACTGCTTCTGTTCTGCAT-3’

55