Primer sets

Primer sequences

Annealing temperature (˚C)

Amplification site (bp)

Rj₂ specific primers

K452E-F

(5'-GCTTCAATAGATATGACTTGACAG-3')

58.5

161

R490I-R

(5'-AATCAAGTCATGCATTGTAACTA-3')

57.8

rj₂ specific primers

K452-F

(5'-GCTTCAATAGATATGACTTGACAA-3')

58.9

161

R490-R

(5'-ATCAAGTCATGCATTGTAACTC-3')

58.2

Rj₄ specific primers

T107A-F

(5'-TTGGAGGAAACGCCG-3')

62.2

324

202-203AY-R

(5'-AATCATGAGAAGAACAAGTATAAGC-3')

58.5

rj₄ specific primers

T107-F

(5'-TTGGAGGAAACGCCA-3')

59.9

318

202-203del-R

(5'-AATCATGAGAAGAACAAGTATGGA-3')

60.4