| Time, min |
Pump A (%), aqueous buffer |
Pump B (%) acetonitrile |
Flow rate, mLmin−1 |
| 0 |
90 |
10 |
0.5 |
| 0.5 |
90 |
10 |
0.5 |
| 1 |
80 |
20 |
0.5 |
| 6 |
20 |
80 |
0.5 |
| 8.5 |
20 |
80 |
0.5 |
| 8.55 |
90 |
10 |
0.75 |
| 10.55 |
90 |
10 |
0.75 |
| 10.6 |
90 |
10 |
0.5 |