Time, min | Pump A (%), aqueous buffer | Pump B (%) acetonitrile | Flow rate, mLmin−1 |
0 | 90 | 10 | 0.5 |
0.5 | 90 | 10 | 0.5 |
1 | 80 | 20 | 0.5 |
6 | 20 | 80 | 0.5 |
8.5 | 20 | 80 | 0.5 |
8.55 | 90 | 10 | 0.75 |
10.55 | 90 | 10 | 0.75 |
10.6 | 90 | 10 | 0.5 |