| USP criteria | Specifications | Day 1 | Day 2 | Day 3 | Pass/Fail |
| Retention time | RSD ≤ 2.0% | 1.3 | 1.3 | 1.3 | Pass |
| Capacity factor k’ | >1.0 | 1.6 | 1.6 | 1.6 | Pass |
| Area | RSD ≤ 2.0% | 0.2 | 0.2 | 0.2 | Pass |
| Theoretical plates | >2000 | 4208 | 4238 | 4215 | Pass |
| USP tailing | <2.0 | 1.8 | 1.8 | 1.8 | Pass |
| Resolution* | >2.0 | 3.2 | 3.3 | 3.4 | Pass |