Test

Observation

Inference

Simple sugar

a) Molish’s test

b) Fehling’s test

Purple colour observed at the interface

Brick red precipitate formed

+

+

Alkaloids

a) Mayer’s test

b) Dragendoff’s test

c) Wagner’s test

Cream precipitate formed

Reddish brown precipitate formed

Reddish brown precipitate

++

++

++

Saponin

Frothing test

Frothing formed

+

Cardiac glycosides

a) Keller-killani’s test

b) Salkowski’s

Brown ring colour is formed

Reddish-brown colour at the interface formed

+

++

Polyphenol

Ferric chloride

Colour change observed

+

Tanins

Ferric chloride

Brownish green precipitate

+