Gene

Primer sequence

Expected size (bp)

annealing temp (˚C)

^BlaTEM

F = 5’GCGGAACCCCTATTTG3’

R = 5’TCTAAAGTATATATGAGTAAACTTGGTCTGAC3’

999 bp

52

^BlaSHV

F = 5’TTCGCCTGTGATTATCTCCCTG3’

R = 5’TTAGCGTTTGCCAGTGYCG3’

851 bp

60

^BlaCTX-M

F = 5’ATGTGCAGYACCAGTAARGTKATGGC3’

R = 5’TGGGTRAARTAARTARGTSACCAGAAYCAGCGG3’

593 bp

60

^BlaOXA

F = 5’ATGAAAAACACAATACATATCAACTTCGC3’

R = 5’GTGTGTTTAGAATGGTGATCGCATT3’

920 bp

52