Virulence genes

PCR condition

(Amplification 35 cycle)

Primary Denaturation

Denaturation

Annealing

Extension

Final extension

Rpt2

95˚C/3min

95˚C/1min

48˚C/1min

72˚C/1min

72˚C/5min

ssa

95˚C/3min

95˚C/1min

48˚C/1min

72˚C/1min

72˚C/5min

FbpA

95˚C/5min

95˚C/30s

50˚C/30s

72˚C/1min

72˚C/7min

lktC

94˚C/5min

94˚C/30s

58˚C/40s

72˚C/40s

72˚C/10min

TbpA

95˚C/5min

95˚C/30s

50˚C/30s

72˚C/1min

72˚C/7min

sodA

95˚C/5min

95˚C/30s

55˚C/30s

72˚C/40s

72˚C/5min

Kmt1

95˚C/5min

95˚C/45s

55˚C/1min

72˚C/1min

72˚C/10min

toxA

94˚C/5min

94˚C/30s

48˚C/40s

72˚C/45s

72˚C/10min

hyaD-hyaC

95˚C/5min

95˚C/1min

55˚C/1min

72˚C/1:30s

72˚C/7min

dcbF

94˚C/5min

94˚C/1min

55˚C/1min

72˚C/30s

72˚C/7min

pmSLP

94˚C/2min

94˚C/15s

62˚C/30s

68˚C/1min

68˚C/5min