Virulence genes | PCR condition (Amplification 35 cycle) | ||||
Primary Denaturation | Denaturation | Annealing | Extension | Final extension | |
Rpt2 | 95˚C/3min | 95˚C/1min | 48˚C/1min | 72˚C/1min | 72˚C/5min |
ssa | 95˚C/3min | 95˚C/1min | 48˚C/1min | 72˚C/1min | 72˚C/5min |
FbpA | 95˚C/5min | 95˚C/30s | 50˚C/30s | 72˚C/1min | 72˚C/7min |
lktC | 94˚C/5min | 94˚C/30s | 58˚C/40s | 72˚C/40s | 72˚C/10min |
TbpA | 95˚C/5min | 95˚C/30s | 50˚C/30s | 72˚C/1min | 72˚C/7min |
sodA | 95˚C/5min | 95˚C/30s | 55˚C/30s | 72˚C/40s | 72˚C/5min |
Kmt1 | 95˚C/5min | 95˚C/45s | 55˚C/1min | 72˚C/1min | 72˚C/10min |
toxA | 94˚C/5min | 94˚C/30s | 48˚C/40s | 72˚C/45s | 72˚C/10min |
hyaD-hyaC | 95˚C/5min | 95˚C/1min | 55˚C/1min | 72˚C/1:30s | 72˚C/7min |
dcbF | 94˚C/5min | 94˚C/1min | 55˚C/1min | 72˚C/30s | 72˚C/7min |
pmSLP | 94˚C/2min | 94˚C/15s | 62˚C/30s | 68˚C/1min | 68˚C/5min |