Primer name

Primer sequences 5'-3'

Denaturation (˚C/sec)

Annealing (˚C/sec)

Elongation (˚C/sec)

Cycle

PCR products

CTX-M-1 group

F-GCTGTTGTTAGGAAGTGTGC

94/60

55/60

72/90

30

516

R-CCATTGCCCGAGGTGAAG

CTX-M-2 group (*)

F-ACGCTACCCCTGCTATTT

94/60

55/60

72/90

30

779 or 780

R-CCTTTCCGCCTTCTGCTC

CTX-M-9 group (**)

F-GCAGATAATACGCAGGTG

94/60

55/60

72/90

30

393

R-CGGCGTGGTGGTGTCTCT

TEM group (***)

F-CCGTGTCGCCCTTATTCC

94/60

55/60

72/90

30

824

R-AGGCACCTATCTCAGCGA

SHV group (****)

F-ATTTGTCGCTTCTTTACTCGC

94/60

55/60

72/90

30

1051

R-TTTATGGCGTTACCTTTGACC

ampC

F-GGGGCGGTTTCTCATGCAGCCAACG

94/60

55/60

72/90

30

1313

R-GAAGCGCTCATGGCACCATCATAGCC

(*) only in A-12

95/30

63/30

72/60

(**) only in D-18

94/60

58/60

72/120

35

(***) only in A-12

95/30

63/30

72/60

(***) only in D-18

95/30

61/30

72/60

(****) only in A-12

95/30

63/30

72/60