| Gram Staining | Biochemical reaction | Presumptive Bacteria | |||||||||||||||
| EMB plate | KIA | MIU | Simon’s Citrate | VP test | Oxidase | Catalase | Mannitol | Starch hydrolysis | Methyl Red | Glucose | Lactose fermentation test | ||||||
| Slunt | Bud | Gas | Motility | Indole | Urease | ||||||||||||
| -Ve | + | A | A | + | + | + | + | − | − | − | + | A | − | + | AG | + | E. coli |
| -Ve | − | K | A | G | + | + | − | + | − | + | + | + | + | + | + | − | Vibrio spp. |
| -Ve | − | K | A | − | − | − | − | − | − | − | + | − | − | + | + | − | Shigella spp. |
| -Ve | − | K | A | G | + | − | + | + | − | − | − | A | − | + | − | − | Salmonella spp. |