Buffer condition | 25 mM HEPES, 100 mM NaCl, pH 8.0 | ||
Temperature (˚C) | 4 | 4 | |
Results | |||
Elution volume (mL) | 14.75 | 15.24 | |
Estimated mass (kDa) | 55.97 | 45.37 | |
Mass Determination | |||
Protein sample | PBP4* | PBP4* ΔCTD | |
Calculated monomeric mass(kDa) (from the sequence, using Expasy software) | ~51 | ~41 | |
Mass (kDa) (from DLS) | 54.5 ± 1.22 | 35.17 ± 1.17 | |
Mass (kDa) (from size-exclusion chromatography) | 55.97 | 45.37 | |
Oligomeric state in solution | Monomeric | Monomeric | |