Primer name

Sequence (5’ to 3’)

pbpE_frag1_fw

GCGCATATGAAACAGAATAAAAGAAAGCATCTTCAGACA

pbpE_frag1_mut1_rev

GTACATCATACACATATCCATAAGCATAATGATCA

pbpE_frag2_mut1_fw

TGATCATTATGCTTATGGATATGTGTATGATGTAC

pbpE_frag2_mut2_rev

GGGACCTCATAAGGCTGGCCAAATA

pbpE_frag3_mut2_fw

TATTTGGCCAGCCTTATGAGGTCCC

pbpE_frag3_rev

ACAACTCGAGATTAATTTGTACGGACCGCTTCTTCTTCT

pbpENterm-fw

GCGCATATGAAACAGAATAAAAGAAAGCATCTTC

pbpENterm-rev

ACAACTCGAGATTAAGCAGGACGTTCCGGG