10 × PCR buffer solution

2.5 μL

MgCL₂ (2.5 mmol/L)

5 μL

dNTPs (10 mM)

2 μL

forward primer (10 umol/L)

1 μL

reverse primer (10 umol/L)

1 μL

probe (5 umol/L)

1 μL

DNA

2 μL

Taqenzyme

0.5 μL

ddH₂O

10 μL