| 10 × Reverse transcriptase buffer | 2 μL |
| MgCL2 (25 mmol/L) | 2 μL |
| dNTPs (10 mM) | 2 μL |
| DTT (0.1 M) | 2 μL |
| reverse primer (10 umol/L) | 4 μL |
| AMV Reverse transcriptase (200 units/µl) | 0.5 μL |
| ddH2O | 7.5 μL |