| PCR | Gene | Primer | Nucleotide sequence（5’ → 3’） | Product size (bp) |
| Multiplex I | Class A β-lactamase | |||
| blaCTX-M-3 | F | AATCACTGCGCCAGTTCACGCT | 479 | |
| R | GAACGTTTCGTCTCCCAGCTGT | |||
| blaCTX-M-14 | F | TACCGCAGATAATACGCAGGTG | 355 | |
| R | CAGCGTAGGTTCAGTGCGATCC | |||
| blaSHV | F | AACGGAACTGAATGAGGCGCT | 141 | |
| R | TCCACCATCCACTGCAGCAGCT | |||
| blaTEM | F | GAAGATCAGTTGGGTGCACGAGT | 520 | |
| R | CAACTTTATCCGCCTCCATCCAGT | |||
| Class C β-lactamase | ||||
| blaDHA | F | AACTTTCACAGGTGTGCTGGGT | 405 | |
| R | CCGTACGCATACTGGCTTTGC | |||
| Simplex | blaCMY-2 | F | CTGACAGCCTCTTTCTCCACA | 1100 |
| R | CTACGTAGCTGCCAAATCCAC | |||
| Multiplex II | hlyA | F | AACAACGATAAGCACTGTTCTGG | 1177 |
| R | CCATATAAGCGGTCATTCCCG | |||
| usp | F | GGAAAATGGTCGCTCAGTGG | 992 | |
| R | CTGTAGTGAATCTCATCGTGTAGTC | |||
| sat | F | TCAGAAGCTCAGCGAATCATTG | 931 | |
| R | CATTATCACCAGTAAAACGCACC | |||
| fyuA | F | GGCTTTATCCTCTGGCCTT | 878 | |
| R | GAAAACCCAGTCATCGGTGG | |||
| iron | F | CTCTGGTGGTGGAAGCC | 815 | |
| R | TGTCGGTACAGGCGGTTC | |||
| iutA | F | CACTCCGGTACTCCAGTCA | 688 | |
| R | CCTCCAACCAGATGTTCTTCG | |||
| iucD | F | CCGGAGAAGCCTGAAATATATTCA | 584 | |
| R | CCGGATTGTCATATGCAGACC | |||
| Multiplex III | fimH | F | GTTTATAATTCGAGAACGGATAAGCC | 491 |
| R | GTGCATAATTTGCCGTTAATCCC | |||
| cnf1 | F | TTCTTCTGTACTTCCCCCAG | 408 | |
| R | TGAGCGGCATCTACTATGAAGT | |||
| traT | F | CATAACCACGGTTCAGCCATC | 328 | |
| R | TTGCACTGGTCAGTTCCAC | |||
| kpsMTII | F | CATCAGACGATAAGCATGAGCA | 269 | |
| R | TGCGCATTTGCTGATACTGT | |||
| papG II | F | GGGCCCCCAAGTAACTC | 189 | |
| R | GGATGAGCGGGCCTTTG | |||