| Plasmids | Replicative | Size (kb) | Amount (ng) | Selection | Transformation efficiency (transformants/μg DNA) | |||
| Recovery time (h) | ||||||||
| 0 | 1 | 2 | 3 | |||||
| pDN19 | Yesa | 7.8 kb | 200 | Tcr | 2.3 × 103 | 1.7 × 107 | 9.6 × 107 | 6.3 × 107 |
| pRKaraRed | Yesa | 10.9 kb | 200 | Tcr | 1.4 × 103 | 4.0 × 106 | 6.8 × 107 | 3.4 × 107 |
| pUFR027 | Yesa | 9.3 kb | 200 | Kanr | 1.9 × 103 | 5.6 × 106 | 3.5 × 107 | 4.9 × 107 |
| pK18mobsacB-Xc0751 | Nob | 7.2 kb | 1000 | Kanr | 6 | 64 | 151 | 136 |
| pK18mobsacB-Xc2728 | Nob | 7.2 kb | 1000 | Kanr | 8 | 79 | 138 | 142 |