Strains and plasmids

Genotype or description

Source

Strains

E. coli DH5α

SupE44 DlacU169 (Φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1

[13]

E. coli S17-1(λpir)

recA, pro, hsdR⁻M⁺RP4: 2-Tc:Mu: Km Tn7 λpir (ATCC 47055)

[14]

Xcc 8004

A rifampicin-resistant strain derived from Xcc NCPPB No. 1145 (isolated from infected cauliflower, B. oleraceavar. botrytis, in Sussex, UK, 1958)

[15]

Plasmids

pDN19

7.8 kb, IncP, oriT, Tc^r

[16]

pUFR027

9.3 kb, IncW, MobP, Ap^r, Kan^r

[17]

pRKaraRed

10.9 kb, IncP, oriT, Tc^r

[18]

pK18mobsacB

5.7 kb, RP4 oriT, Kan^r

[19]

pEX18Gm

Gene replacement vector, oriT, Ap^r

[9]

pK18mobsacB-Xc1075

7.2 kb, pK18mobsacB with the upstream 500 bp fragment of XC1075, accC1 and the downstream 500 bp fragment of XC1075, Kan^r

This work

pK18mobsacB-Xc2659

7.2 kb, pK18mobsacB with the upstream 500 bp fragment of XC2659, accC1 and the downstream 500 bp fragment of XC2659, Kan^r

This work