Strain

Relevant genotype

Expressed efflux

Antibiotic marker

MIC (mg/ml)

Efflux determined by Multiplex RT-PCR

−PAβN

+PAβN

Reduction fold

PAO1

Wild-type

MexAB-OprM

Carbenicillin

128

16

8

MexAB-OprM

PAO200

PAO1D(mexAB-oprM)

None

1

1

0

None

PAO1

Wild-type

MexAB-OprM

Erythromycin

512

256

2

Wild-type

PAO200

PAO1D(mexAB-oprM)

None

8

8

-

None

PAO200-2

PAO200nfxB

MexCD-OprJ

512

4

128

MexCD-OprJ

PAO238

PAO200-2D(mexCD-oprJ)

None

32

4

8

None

PAO7H1A

PAO7H^aD(mexAB-oprM)

MexEF-OprN

Imipenem

8

4

2

MexEF-OprN

PAO255

PAO7H1AD(mexEF-oprN)

None

1

0.5

2

None

PAO7H1A

PAO7HD(mexAB-oprM)

MexEF-OprN

Norfloxacin

2

0.06

33

MexEF-OprN

PAO255

PAO7H1AD(mexEF-oprN)

None

<0.06

<0.06

-

None

PAO267

PAO3579^b with D(mexAB-oprM)

MexXY

Gentamicin

2

0.03

64

MexXY

PAO280

PAO267 with D(mexXY)

None

2

0.25

8

None