Assay

Variables

Responses

Coded values

Real values

X₁

X₂

Agitation (rpm)

Aeration^*

Cell (g/L)

Lipid (g/L)

Consumption of glycerol (%)

Y_P/S

(g/g)

Y_x/S

(g/g)

Q_P

(g/L∙day)

1

−1

−1

150

2.5

10.5

0.89

33.3

0.038

0.45

0.13

2

1

−1

250

2.5

24.2

2.32

74.4

0.044

0.47

0.47

3

−1

1

150

5.0

18.0

1.65

64.7

0.037

0.40

0.22

4

1

1

250

5.0

39.4

4.45

87.6

0.073

0.64

0.95

5

−1

0

150

3.75

16.8

1.24

54.1

0.033

0.44

0.21

6

1

0

250

3.75

30.9

4.18

81.0

0.074

0.54

0.80

7

0

−1

200

2.5

14.9

1.54

46.3

0.048

0.46

0.30

8

0

1

200

5.0

27.6

3.62

73.1

0.071

0.54

0.65

9

0

0

200

3.75

21.5

1.60

68.4

0.033

0.45

0.27

10

0

0

200

3.75

18.9

1.21

69.6

0.025

0.39

0.23

11

0

0

200

3.75

19.6

1.06

58.3

0.026

0.48

0.19