| Sample No | Spiked level (%) | Amount of Bimatoprost added in µg | Amount of Bimatoprost found in mg | % of Recovery | Mean & % RSD |
| 1 | 50 | 13768.00 | 13887.78 | 100.87 | 100.01% & 0.19% |
| 2 | 50 | 13728.00 | 13895.48 | 101.22 | |
| 3 | 50 | 13752.00 | 13879.89 | 100.93 | |
| 4 | 100 | 27304.00 | 27353.15 | 100.18 | 100.11% & 0.07% |
| 5 | 100 | 27320.00 | 27333.66 | 100.05 | |
| 6 | 100 | 27320.00 | 27350.05 | 100.11 | |
| 7 | 150 | 40968.00 | 41201.52 | 100.57 | 100.59% & 0.03% |
| 8 | 150 | 41000.00 | 41254.20 | 100.62 | |
| 9 | 150 | 41016.00 | 41253.89 | 100.58 | |
| Grand average (%) | 100.57% | ||||
| RSD of nine determinations | 0.40% | ||||
| 95% confidence interval | 100.57 ± 0.26 | ||||