Sample No

Spiked level (%)

Amount of Bimatoprost added in µg

Amount of Bimatoprost found in mg

% of Recovery

Mean & % RSD

1

50

601.60

592.22

98.44

98.92%

& 0.42%

2

50

600.00

594.96

99.16

3

50

600.80

595.75

99.16

4

100

1204.80

1210.46

100.47

99.75%

& 0.79%

5

100

1205.60

1192.34

98.90

6

100

1201.60

1200.16

99.88

7

150

1832.00

1817.34

99.20

99.90%

& 0.73%

8

150

1816.00

1827.80

100.65

9

150

1824.00

1821.26

99.85

Grand average (%)

99.52%

RSD of nine determinations

0.74%

95% confidence interval

99.52 ± 0.48