Sample No | Spiked level (%) | Amount of Bimatoprost added in µg | Amount of Bimatoprost found in mg | % of Recovery | Mean & % RSD |
1 | 50 | 601.60 | 592.22 | 98.44 | 98.92% & 0.42% |
2 | 50 | 600.00 | 594.96 | 99.16 | |
3 | 50 | 600.80 | 595.75 | 99.16 | |
4 | 100 | 1204.80 | 1210.46 | 100.47 | 99.75% & 0.79% |
5 | 100 | 1205.60 | 1192.34 | 98.90 | |
6 | 100 | 1201.60 | 1200.16 | 99.88 | |
7 | 150 | 1832.00 | 1817.34 | 99.20 | 99.90% & 0.73% |
8 | 150 | 1816.00 | 1827.80 | 100.65 | |
9 | 150 | 1824.00 | 1821.26 | 99.85 | |
Grand average (%) | 99.52% | ||||
RSD of nine determinations | 0.74% | ||||
95% confidence interval | 99.52 ± 0.48 |