| Treatment condition | Sample Name | % of recovery | Remarks |
| Without Treatment | Placebo | N/A | Not degraded |
| Sample | Bimatoprost: 99.60% and Timolol: 99.84% | Not degraded | |
| Acid hydrolysis of Bimatoprost (treated with 0.2 ml of 1N HCl at 85˚ for 15 min) | Placebo | N/A | Not degraded |
| Standard | 88.21 | Bimatoprost peak is degraded | |
| Sample | 88.66 | Bimatoprost peak is degraded | |
| Acid hydrolysis of Timolol (treated with 1ml of 1N HCl at 85˚ for 11 hours) | Placebo | N/A | Not degraded |
| Standard | 89.79 | Timolol peak is degraded | |
| Sample | 89.52 | Timolol peak is degraded | |
| Base hydrolysis of Bimatoprost (treated with 10 ml of 1N NaOH at 85˚ for 1.5 hours) | Placebo | N/A | Not degraded |
| Standard | 93.00 | Bimatoprost peak is degraded; | |
| Sample | 93.65 | Bimatoprost peak is degraded; | |
| Base hydrolysis of Timolol (treated with 0.5 ml of 1N NaOH at 85˚ for 1.5 hours) | Placebo | N/A | Not degraded |
| Standard | 87.55 | Timolol peak is degraded | |
| Sample | 87.62 | Timolol peak is degraded | |
| Peroxide oxidation of Bimatoprost (0.5 ml of 30% H2O2 heated at 85˚C for 50 minutes | Placebo | N/A | Not degraded |
| Standard | 90.35 | Bimatoprost peak is degraded | |
| Sample | 90.58 | Bimatoprost peak is degraded | |
| Peroxide oxidation of Timolol (0.5 ml of 30% H2O2 heated at 85˚C for 15 minutes | Placebo | N/A | Not degraded |
| Standard | 79.38 | Timolol peak is degraded | |
| Sample | 79.63 | Timolol peak is degraded | |
| Thermal degradation of Bimatoprost heat at 85˚C for 4.5 hours | Placebo | N/A | Not degraded |
| Standard | 82.26 | Bimatoprost peak is degraded | |
| Sample | 81.99 | Bimatoprost peak is degraded | |
| Thermal degradation of Timolol (heat at 85˚C for 19 hours) | Placebo | N/A | Not degraded |
| Standard | 98.18 | Timolol peak is not degraded | |
| Sample | 98.35 | Timolol peak is not degraded | |
| Photolytic degradation of Bimatoprost (exposed to direct sunlight for 02 hours) | Placebo | N/A | Not degraded |
| Standard | 94.07 | Bimatoprost peak is degraded | |
| Sample | 94.61 | Bimatoprost peak is degraded | |
| Photolytic degradation of Timolol (exposed to direct sunlight for 02 hours) | Placebo | N/A | Not degraded |
| Standard | 86.64 | Timolol peak is degraded | |
| Sample | 86.80 | Timolol peak is degraded |