| Sample Number | Amount of Timolol added in µg | Amount of Timolol found in µg | % of Assay | Average Result (%) | RSD (%) | 95% confidence Interval |
| 1 | 27320.00 | 27229.84 | 99.67 | 99.78 | 0.27 | 99.78 ± 0.22 |
| 2 | 27320.00 | 27284.48 | 99.87 | |||
| 3 | 27320.00 | 27388.30 | 100.25 | |||
| 4 | 27320.00 | 27218.92 | 99.63 | |||
| 5 | 27320.00 | 27259.90 | 99.78 | |||
| 6 | 27320.00 | 27177.94 | 99.48 |