Chromatographic Conditions | |||||
S. No. | Analytical Column | Mobile phase composition | Flow rate | Retention time (min) | Result |
1) | Zorbex SB phenyl, L11 (4.6 mm × 250 mm) 5 µm | pH 2.8 phosphate buffer, methanol and acetonitrile at a ratio of (50:30:20) | 0.9 ml/min | 11.99 min & 4.18 min | Improper peak separation and more RT |
2) | Zorbex SB phenyl, L11 (4.6 mm × 250 mm) 5 µm | pH 2.8 phosphate buffer, methanol and acetonitrile at a ratio of (50:30:20) | 1.0 ml/min | 10.81 min & 3.77 min | More RT but pack separation, theatrical plate and trailing factor was good. |
3) | Zorbex SB phenyl, L11 (4.6 mm × 250 mm) 5 µm | pH 2.8 phosphate buffer, methanol and acetonitrile at a ratio of (50:30:20) | 1.1 ml/min | 9.84 min & 3.43 min | Improper peak separation and more RT |
4) | Zorbex SB phenyl, L11 (4.6 mm × 250 mm) 5 µm | pH 2.7 phosphate buffer, methanol and acetonitrile at a ratio of (50:30:20) | 1.0 ml/min | 10.74 min & 3.76 min | Improper peak separation and more RT |
5) | Zorbex SB phenyl, L11 (4.6 mm × 250 mm) 5 µm | pH 2.9 phosphate buffer, methanol and acetonitrile at a ratio of (50:30:20) | 1.0 ml/min | 10.36 min & 3.74 min | Improper peak separation and more RT |
6) | Zorbex SB phenyl, L11 (4.6 mm × 250 mm) 5 µm | 48:52 Mobile Phase Ratio | 1.0 ml/min | 8.78 min & 3.58 min | Improper peak separation, more tailing and less plate count |
7) | Zorbex SB phenyl, L11 (4.6 mm × 250 mm) 5 µm | 52:48 Mobile Phase Ratio | 1.0 ml/min | 12.13 min & 3.89 min | Improper peak separation and more RT |
8) | Phenomenex C18 ODS (4.6 × 150 mm) 5 µm | pH 2.8 phosphate buffer, methanol and acetonitrile at a ratio of (50:30:20) | 1.0 ml/min | 7.8 min & 3.42 min | Improper peak separation, more tailing and less plate count |
9) | Inertsil C18 (15 cm × 0.46 cm) 5 µm | pH 2.8 phosphate buffer, methanol and acetonitrile at a ratio of (50:30:20) | 1.0 ml/min | 14.2 min & 4.9 min | Improper peak separation and more RT |