| Parameters | Peak Area | Retention Time (min) | Theoretical plates | Peak Asymmetry |
| Optimized flow rate of 1.0 mL∙min−1 | 648,759 | 5.484 | 6845 | 1.08 |
| At 0.9 mL∙min−1 flow conditions | 635,248 | 5.599 | 6786 | 1.09 |
| At 1.1 mL∙min−1 flow conditions | 659,865 | 4.576 | 6528 | 1.05 |
| Lesser percent organic solvent | 625,986 | 7.415 | 6689 | 1.03 |
| Higher percent organic solvent | 615,869 | 3.827 | 6354 | 1.01 |