| S. Nо. | Concentration (µg/ml) | Peak Area |
| 1 | 6 | 468,784 |
| 2 | 8 | 615,798 |
| 3 | 10 | 768,759 |
| 4 | 12 | 925,748 |
| 5 | 14 | 1,078,765 |
| Mean | - | 771,570.8 |
| SD | - | 216,375.5 |