Trials | Chromatographic conditions | ||||
S. No. | Analytical Column | Mobile phase composition | Flow rate | Retention time (min) | Result |
1 | Inertsil C18 (15 cm × 0.46 cm) 5 µm | Water: Methanol (50:50% v/v) | 0.8 ml/min | 7.553 | Improper peak separation and more RT. |
2 | Zodiac C18 (4.6 × 150 mm) 5 µm | ACN: MeOH (40:60%) v/v | 0.9 ml/min | 2.508 | Improper peak separation and less plate count. |
3 | Develosil C18 (4.6 × 150 mm) 5 µm | ACN: 0.1% OPA (pH-3.6) (30:70) v/v | 1.0 ml/min | 3.213 | Improper peak separation and less plate count. |
4 | Phenomenex C18 ODS (4.6 × 150 mm) 5 µm | ACN: Ammonium buffer (0.1 M) (45:55% v/v) | 1.0 ml/min | 3.213 | Improper peak separation, more tailing and less plate count. |
5 | Symmetry C18 ODS (4.6 × 150 mm) 5 µm | MeOH: Phosphate buffer (0.01 M) (40:60%) v/v | 1.0 ml/min | 4.161 | Improper peak separation and peak broadening. |
6 | Symmetry C18 ODS (4.6 × 150 mm) 5 µm | MeOH: Phosphate buffer (0.05 M) (20:80%) v/v | 1.0 ml/min | 4.396 | Improper peak separation, broad peak and peak tailing. |