Parameters

Studied range

Selected value

Excitation wavelength maximum/λ_ex (nm)

200 - 700

319

Emission wavelength maximum/λ_em (nm)

200 - 700

371

Solvent/amount of ethanol/mL

0 - 5

2 - 5

(Preferably 2)

Acidity/M H₂SO₄/mL

0.0005 - 0.02

0.0035 - 0.0085

(Preferably 0.005)

pH

3.30 - 1.70

2.35 - 2.07

(Preferably 2.30)

Time/h

0 - 72

1 min - 24 h

(Preferably 5)

Temperature/˚C

10 - 70

15 - 50

(Preferably 25 ± 5)

Reagent

(fold molar excess, M:R)

1:50 - 1:400

1:130 - 1:300

(Preferably 1:150)

Linear range/µg∙L⁻¹

0.0001 - 1000

0.001 - 600

Limit of quantization/ng∙L⁻¹

0.1 - 100

3.0

Detection limit/ng∙L⁻¹

0.01 - 10.0

0.3

Reproducibility (% RSD)

0 - 10

0 - 2%

Regression Coefficient (R²)

0.9995 - 0.9999

0.9998