Meth 1

Meth 2

AM

AM

CL

Response function

(p = 4, n = 3)

(p = 4, n = 3 )

(p = 4, n = 3 )

Linear regression

Through 0 and level 3

Through 0 and level 3

Through 0 and level 3

Trueness

Absolute biais (µg∙mL⁻¹)

Relative biais (%)

Absolute biais (µg∙mL⁻¹)

Relative biais (%)

Absolute biais (µg∙mL⁻¹)

Relative biais (%)

Level 1

0.46

0.92

−0.10

−0.21

−0.01

−0.09

Level 2

−0.50

−0.66

0.33

−0.43

−0.03

−0.31

Level 3

−0.86

−0.86

0.28

0.28

0.02

0.14

Level 4

−1.38

−1.10

−1.03

−0.85

−0.18

−0.18

Level 5

−3.40

−2.26

−0.39

−0.26

−0.12

−0.63

Precision

Repeatability (%RSD)

Intermediate precision (%RSD)

Repeatability (%RSD)

Intermediate precision (%RSD)

Repeatability (%RSD)

Intermediate precision (%RSD)

Level 1

0.45

1.50

0.20

0.89

0.14

0.73

Level 2

1.61

1.63

0.17

1.53

0.11

1.08

Level 3

0.35

0.90

0.18

1.12

0.17

0.86

Level 4

0.68

1.57

0.18

1.45

0.19

1.40

Level 5

0.52

2.82

0.17

1.43

0.24

1.49

Accuracy

Relative β-expectation tolerance limits (%)

Relative β-expectation tolerance limits (%)

Relative β-expectation tolerance limits (%)

Level 1

[−4.31; 5.89]

[−3.24; 2.82]

[−2.61; 2.44]

Level 2

[−4.44; 3.12]

[−5.80; 4.94]

[−4.14; 3.51]

Level 3

[−3.76; 2.04]

[−3.63; 4.19]

[−2.83; 3.12]

Level 4

[−5.96; 3.75]

[−5.91; 4.26]

[−5.92; 3.56]

Level 5

[−12.02; 7.49]

[−5.30; 4.78]

[−5.82; 4.57]

Linearity

Y = 2.306 + 0.966X

Y = 0.199 + 0.995X

Y = 0.088 + 0.988X

Range

(µg∙mL⁻¹)

[50; 142]

[50; 150]

[6.24; 18.71]

R2

0.997

0.999

0.999

LOQ in (µg∙mL⁻¹)

LOD

LLOQ

ULOQ

LOD

LLOQ

ULOQ

LOD

LLOQ

ULOQ

1.15

50

141.7

0.50

50

150

0.08

6.24

18.71