Original sample

Saponification

Heptane extract

EtOH: H₂O acid wash

Rⁱⁱⁱ-O-R^iv

Rⁱⁱ-OH

RCOOR

Rⁱⁱⁱ-O-R^iv

Rⁱⁱ-OH

RCOO⁻

R¹OH

Rⁱⁱⁱ-O-R^iv

Rⁱⁱ-OH

RCOO residual

R¹OH

NaOH residual

Rⁱⁱⁱ-O-R^iv

Rⁱⁱ-OH

RCOOH^1,3

R¹OH

NaCl

HCl²