| R1: p-value | R2: p-value | R3: p-value | R4: p-value | R5: p-value | |
| Source | Prob > F | Prob > F | Prob > F | Prob > F | Prob > F |
| Model | 0.0018 | 0.0184 | 0.1445 | <0.0001 | 0.0023 |
| A: Flow | 0.1732 | 0.0064 | 0.0442 | <0.0001 | 0.0008 |
| B: Temperature | 0.0011 | 0.0052 | 0.0862 | <0.0001 | 0.0007 |
| C: Mobile phase A pH | 0.0003 | 0.0383 | 0.0862 | <0.0001 | 0.0034 |
| AB | 0.3473 | 0.1747 | 0.7575 | <0.0001 | 0.0987 |
| AC | 0.6657 | 0.7194 | 0.4 | <0.0001 | 0.1748 |
| BC | 0.0346 | 0.4365 | 0.7575 | <0.0001 | 0.42 |
| ABC | 0.3064 | 0.3021 | 0.4 | <0.0001 | 0.6509 |
| Curvature | 0.0665 | 0.1663 | 0.4534 | <0.0001 | 0.0149 |