| Method parameters (factors) | Units | (Old) Target | (Revised) Target | Operational range | |
| LOW | High | ||||
| Flow | mL/min | 1.0 | 1.2 | 0.8 | 1.2 |
| Temperature | ˚C | 30 | 35 | 25 | 35 |
| Mobile phase A pH | N/A | 4.5 | 4.7 | 4.4 | 4.7 |