Parameter

Modification

Retention time (min)

Tailing factor

Theoretical plates

Resolution

DM

PH

DM

PH

DM

PH

DM & PH

Mobile phase ratio (v/v),

acetonitrile:buffer (pH 3.1)

60:40

6.36

7.94

1.02

1.04

1722

1822

3.2

62:38

5.88

6.43

1.01

0.96

1719

1835

3.5

64:36

4.84

6.02

1.03

1.04

1421

1254

3.1

Flow rate (mL∙min⁻¹)

0.8

6.35

7.92

1.02

1.13

2748

2684

3.6

1.0

5.82

6.42

1.11

1.04

1123

1738

2.9

1.2

4.84

5.12

0.94

1.12

2692

2103

3.0

Buffer concentration (mM)

10

6.35

7.94

1.12

1.09

1114

1836

3.3

5

6.12

7.26

1.10

0.98

1816

1847

3.6

15

4.93

6.17

1.03

1.02

1919

1843

3.8

pH

10

6.36

7.94

1.00

1.02

1831

1812

3.2

5

6.23

7.88

1.04

1.01

1054

1825

3.5

15

6.24

7.83

0.99

1.10

1735

1835

3.6

Temperature in ˚C

35

6.36

7.94

1.15

1.15

1728

1846

3.3

40

5.87

6.45

1.06

1.11

1094

1849

3.8

45

4.87

6.12

1.13

1.13

1635

1746

3.1