Stress conditions applied for Saxagliptin (1000 ng/spot)

Time (h)

% of active drug present after degradation ± SD

Acid (HCl 0.1 N; 80˚C)

1

78.65 ± 1.72

Acid (HCl 1 N; 80˚C)

1

75.23 ± 1.53

Alkali (NaOH 0.1 N; 80˚C)

1

37.08 ± 0.97

Alkali (NaOH 1 N; 80˚C)

1

34.71 ± 1.35

3% H₂O₂

1

70.18 ± 1.03

Photolytic degradation (Sunlight)

24

91.65 ± 1.46