| Matrix | Time (min) | Solvent A (%) | Solvent B (%) | Flow rate (mL/min) |
| Plasma | Initial | 50 | 50 | 0.2 |
| 3.00 | 10 | 90 | 0.2 | |
| 6.00 | 10 | 90 | 0.2 | |
| 6.01 | 50 | 50 | 0.2 | |
| 12.00 | 50 | 50 | 0.2 | |
| Lung homogenate | Initial | 70 | 30 | 0.2 |
| 3.00 | 10 | 90 | 0.2 | |
| 8.00 | 10 | 90 | 0.2 | |
| 8.10 | 70 | 30 | 0.2 | |
| 14.00 | 70 | 30 | 0.2 |