Genes | Primer Code | Nucleotide Sequences (Primers) | Size after PCR | Restriction Enzymes (RE) | Results from RFLP |
Factor VIII (F8) | F8-S | 5’GTGAGTAGCAGTGTGGGCAGA 3' | 608 bp | HindIII | (−): 427 and 181 bp (Wild-type) (+): 427, 100 and 81 bp (Mutant) |
F8-A | 5’CTGAAATGAAACGGGTGGAAC 3' | ||||
Factor IX (F9) | F9-S | 5’GATAGAGAAACTGGAAGTAGACCC 3' | 369 bp | MseI | (−): 83, 73 bp (Wild-type) (+): 57, 73 bp (Mutant) |
F9-A | 5’TTAGGTCTTTCACAGAGTAGAATTT 3' |