Genes

Primer Code

Nucleotide Sequences (Primers)

Size after PCR

Restriction Enzymes (RE)

Results from RFLP

Factor VIII (F8)

F8-S

5’GTGAGTAGCAGTGTGGGCAGA 3'

608 bp

HindIII

(−): 427 and 181 bp

(Wild-type)

(+): 427, 100 and 81 bp

(Mutant)

F8-A

5’CTGAAATGAAACGGGTGGAAC 3'

Factor IX (F9)

F9-S

5’GATAGAGAAACTGGAAGTAGACCC 3'

369 bp

MseI

(−): 83, 73 bp

(Wild-type)

(+): 57, 73 bp

(Mutant)

F9-A

5’TTAGGTCTTTCACAGAGTAGAATTT 3'