RNA Concentration in the spiked volume (in copies)

2.0 × 10²

1.0 × 10²

5 × 10¹

2.5 × 10¹

1.25 × 10¹

6.25 × 10⁰

3.12 × 10⁰

Positives/Total

3/3

3/3

3/3

3/3

3/3

3/3

1/3

Mean Ct

28.76

29.95

31.12

33.36

38.58

35.59

39.38

Standard Deviation (Ct)

0.33

0.29

0.29

0.98

1.56

0.75

NA