RNA Concentration in spiked volume (in copies)

5 × 10¹

2.5 × 10¹

1.25 × 10¹

6.25 × 10⁰

Positives/Total

20/20

20/20

16/20

9/20

Mean Ct

33.40

33.78

35.45

38.31

Standard Deviation (Ct)

1.05

1.41

1.55

1.32