| Replicates | Microchip RT-PCR Ct | IC Target | |
| N1 | N2 | Hs | |
| 1 | 26.16 | 25.97 | 25.02 |
| 2 | 26.11 | 26.03 | 25.42 |
| 3 | 26.08 | 26.60 | 25.28 |
| 4 | 26.29 | 25.87 | 25.19 |
| 5 | 26.27 | 25.92 | 25.22 |
| 6 | 26.10 | 26.02 | 25.52 |
| 7 | 26.30 | 26.11 | 25.50 |
| 8 | 26.09 | 26.03 | 25.60 |
| 9 | 26.15 | 26.05 | 25.53 |
| NTC | 0 | 0 | 0 |
| Mean | 26.17 | 26.07 | 25.36 |
| SD | 0.09 | 0.21 | 0.20 |
| CV% | 0.34 | 0.81 | 0.77 |