Treatment

Volume added (ml)

Standard mutagen (NaN₃)

Herbal extract

Reagent mixture

Water

Salmonella strain TA100

Blank

-

-

2.5

17.5

-

Background

-

-

2.5

17.5

0.005

Standard mutagen

0.1

-

2.5

17.4

0.005

Test sample

-

0.005

2.5

17.5

0.005