Factors

Name of factors

Levels

−α

−1

0

+1

+α

X₁

pH of the reaction mixture

6

7

8

9

10

X₂

Cholesterol concentration

0.2

0.4

0.6

0.8

1.0

X₃

4-Aminoantipyrine

−0.5

0.5

1.0

1.5

2.5

X₄

Crude COX volume

0

50

100

150

200

X₅

Horseradish Peroxidase

0

5

10

15

20