Procedures | Flavonoid Artocarpus odoratissimus | |
Coumpound 1 | Coumpound 2 | |
Extraction | Macerated 460 g dried of A. odoratissimus with methanol turn out 27.19 g brown crude extract | Macerated 460 g dried of A. odoratissimus with methanol turn out 27.19 g brown crude extract |
TLC Cromatogram | n-hexane and chloroform (7:3) as eluent | n-hexane and chloroform (6:4) as eluent |
Fractionation | GCC of 1 g crude extract over Si gel, using a n-hexane and chloroform (7:3) as eluent, afforded 6 fractions (F1 = 172 mg, F2 = 28 mg, F3 = 207 mg, F4 = 119 mg, F5 = 37 mg, F6 = 201 mg) | GCC of 1 g crude extract over Si gel, using a n-hexane and chloroform (7:3) as eluent, afforded 6 fractions (F1 = 172 mg, F2 = 28 mg, F3 = 207 mg, F4 = 119 mg, F5 = 37 mg, F6 = 201 mg) |
Purification | Recrystallization method was used for purification fraction I to yield 106 mg coumpound 1 | Fraction 3 was GCC on Si gel (n-hexane and chloroform (6:4)) to yield 31 mg coumpound 2 |