Obtained Fragment | Restriction Enzyme | Annealing Temperature | Primers 5'→3' | Reference ID | Gene Polymorphism |
D allele→84 bp I allele→64 bp | Taq I | 62˚ | ACE1―CAT CCT TTC TCC CATTTC TC ACE2―TGGGATTACAGGCGTGATAC ACE3―ATTTCAGAGCTGGAATAAAA | rs5186 | ACE I/D |
R allele→250, 86 bp X allele→108, 97 bp | DdeI | 62˚ | F―5' CGCCCTTCAACAACTGGCTGGA 3' R―5' GGGTGATGTAGGGATTGGTGGAG 3' | rs1815739 | ACTN3 R/X |