Parameter

HBH

HWN

Type of test

Conclusion

Effect of husbandry

Ref (s)

Protein/DNA ratio

13 ± 4

18 ± 6

student’s t-testb

HWN > HBH (1.4-fold)

ND

[12]

Sym GCP

16 ± 7

15 ± 6

student’s t-test

HBH = HWN

ND

[12]

Sym gene expression (normalized to the exogenous Solaris® RNA spike & Sym GCP as in Mayfield et al. [55] ; in situ gene expression levels were not determined.)

Sym apx1 (stress response)

2.8 ± 1.4

3.5 ± 2.5

student’s t-test

HBH = HWN

ND

[12]

Sym hsp70 (stress response)

670 ± 270

740 ± 290

student’s t-testb

HBH = HWN

ND

[15]

Sym pgpase (photosynthesis)

12 ± 5.2

13 ± 7.4

student’s t-testc

HBH = HWN

ND

[12]

Sym psI (photosynthesis)

101 ± 50

74 ± 51

student’s t-testb

HBH > HWN (1.5-fold)

ND

[12]

Sym rbcL (photosynthesis)

30 ± 21

40 ± 35

student’s t-testc

HBH = HWN

ND

[12]

Sym nrt2 (metabolism)

750 ± 230

1060 ± 760

student’s t-testb

HBH = HWN

ND

[15]

Host coral gene expression (normalized to the exogenous Solaris® RNA spike & host GCP as in Putnam et al. [56] ; in situ gene expression levels were not determined.)

Host hsp70 (stress response)

75 ± 12

71 ± 11

student’s t-test

HBH = HWN

ND

[15]

Host actb (cytoskeleton)

110 ± 31

107 ± 33

student’s t-testb

HBH = HWN

ND

[15]

Host ezrin (cytoskeleton)

58 ± 35

80 ± 27

student’s t-test

HBH = HWN

ND

[15]

Host trp1 (cytoskeleton)

23 ± 8.2

28 ± 8.5

student’s t-test

HBH = HWN

ND

[15]

Host tuba (cytoskeleton)

230 ± 81

220 ± 51

student’s t-test

HBH = HWN

ND

[15]

Host cplap2 (osmoregulation)

1.5 ± 0.66

2.1 ± 0.78

student’s t-test

HBH = HWN

ND

[15]

Host oatp (osmoregulation)

5.7 ± 2.6

5.4 ± 1.6

student’s t-test

HBH = HWN

ND

[15]

Host trcc (osmoregulation)

210 ± 81

210 ± 76

student’s t-testb

HBH = HWN

ND

[15]

Sym protein expression (western blot-derived; concentration normalized to the Sym GCP)

Sym RBCL (photosynthesis)

320 ± 170

200 ± 94

student’s t-testb

HBH = HWN

ND

[16]

All 23 response variables listed above (standardized)

PERMANOVA

HBH = HWN

ND

herein

Protein expression (2D + MS)

15 proteins across 3 sequenced spots

38 proteins across 6 sequenced spots

See text for details.

See text for details.

ND

Herein &

[17]