Parameter | HBH | HWN | Type of test | Conclusion | Effect of husbandry | Ref (s) |
Protein/DNA ratio | 13 ± 4 | 18 ± 6 | student’s t-testb | HWN > HBH (1.4-fold) | ND | [12] |
Sym GCP | 16 ± 7 | 15 ± 6 | student’s t-test | HBH = HWN | ND | [12] |
Sym gene expression (normalized to the exogenous Solaris® RNA spike & Sym GCP as in Mayfield et al. [55] ; in situ gene expression levels were not determined.) | ||||||
Sym apx1 (stress response) | 2.8 ± 1.4 | 3.5 ± 2.5 | student’s t-test | HBH = HWN | ND | [12] |
Sym hsp70 (stress response) | 670 ± 270 | 740 ± 290 | student’s t-testb | HBH = HWN | ND | [15] |
Sym pgpase (photosynthesis) | 12 ± 5.2 | 13 ± 7.4 | student’s t-testc | HBH = HWN | ND | [12] |
Sym psI (photosynthesis) | 101 ± 50 | 74 ± 51 | student’s t-testb | HBH > HWN (1.5-fold) | ND | [12] |
Sym rbcL (photosynthesis) | 30 ± 21 | 40 ± 35 | student’s t-testc | HBH = HWN | ND | [12] |
Sym nrt2 (metabolism) | 750 ± 230 | 1060 ± 760 | student’s t-testb | HBH = HWN | ND | [15] |
Host coral gene expression (normalized to the exogenous Solaris® RNA spike & host GCP as in Putnam et al. [56] ; in situ gene expression levels were not determined.) | ||||||
Host hsp70 (stress response) | 75 ± 12 | 71 ± 11 | student’s t-test | HBH = HWN | ND | [15] |
Host actb (cytoskeleton) | 110 ± 31 | 107 ± 33 | student’s t-testb | HBH = HWN | ND | [15] |
Host ezrin (cytoskeleton) | 58 ± 35 | 80 ± 27 | student’s t-test | HBH = HWN | ND | [15] |
Host trp1 (cytoskeleton) | 23 ± 8.2 | 28 ± 8.5 | student’s t-test | HBH = HWN | ND | [15] |
Host tuba (cytoskeleton) | 230 ± 81 | 220 ± 51 | student’s t-test | HBH = HWN | ND | [15] |
Host cplap2 (osmoregulation) | 1.5 ± 0.66 | 2.1 ± 0.78 | student’s t-test | HBH = HWN | ND | [15] |
Host oatp (osmoregulation) | 5.7 ± 2.6 | 5.4 ± 1.6 | student’s t-test | HBH = HWN | ND | [15] |
Host trcc (osmoregulation) | 210 ± 81 | 210 ± 76 | student’s t-testb | HBH = HWN | ND | [15] |
Sym protein expression (western blot-derived; concentration normalized to the Sym GCP) | ||||||
Sym RBCL (photosynthesis) | 320 ± 170 | 200 ± 94 | student’s t-testb | HBH = HWN | ND | [16] |
All 23 response variables listed above (standardized) | PERMANOVA | HBH = HWN | ND | herein | ||
Protein expression (2D + MS) | 15 proteins across 3 sequenced spots | 38 proteins across 6 sequenced spots | See text for details. | See text for details. | ND | Herein & [17] |