Primer

Sequence

Product Size (bp)

Amplification region

5' ® 3'

P1

F

TCTGTGGAGTTTCCAGATCT

541

Exon 1

R

AAGCAGTGTGTCCCTTGACA

P2

F

ACCTATGGCCAAATGAACAA

571

Exon 1-3

R

AGTGGACTGGCAAATCCTTC

P3

F

ACCACTATTACGTAATCCTG

549

Exon 2-5

R

AGCCATGGCCAGATACGACT