Set | Primer name1 | Forward (5’→3’) | Reverse(5’→3’) | Product (bp) | Annealing temp. (˚C) |
1 | 5’-flanking region 1 | GGCCAGAACCAAGTCCTC | GGTACGTGTCCGAACTCCTC | 544 | 64 |
2 | 5’-flanking region 2 | TCTTTGTGCGGGCATCAG | CCTGCAAAACTCCTGGGTAG | 428 | 64 |
3 | 5’-flanking region 3 | GGCAGAGGAGGTTTCGAG | GTAGACCTTGCGATCGATGA | 420 | 58 |
4 | Exon 1 | GAAGACAAAAGCCGAAAGGA | CGCTGGAGGTCTTGTCTGA | 375 | 58 |
5 | Exon 2 | AGCCGCAGTAGAGGTCTTTG | TTCCTCATCCGGTCAGACTC | 181 | 58 |
6 | Exon 3 and 4 | TGACTCCTTCCTCGTGCTCT | GTGCCCACCCACTAACTGAT | 500 | 58 |
7 | Exon 5 | CCTGCTCATCCCTGTTCCT | CCCATCCCCTTCATCGTA | 175 | 60 |
8 | Exon 6 and 7 | GGGCTCACAGTCTCTGCTTC | CCTCCTCCCCACACTCCT | 579 | 62 |
9 | Exon 8 and 9 | GCAAGACACTCCCACGTCTA | TCCTGGGGACATGGACCT | 553 | 58 |
10 | Exon 10 | CTTTGTGCTTCTGCTTCCAG | TCCACTGTATGCCCTCCAC | 178 | 64 |
11 | Exon 11 | AGAGGCGGACAGGGTCTC | ATGCCCACCCCTCCTTAC | 238 | 62 |
12 | Exon 12 and 3’-untranslated region 1 | CATCCATTTCCTCCTCTTCC | GGCCCTGGACATCTGAAG | 599 | 58 |
13 | 3’-untranslated region 2 | TTCTGTGGGGTGATGATGTG | TTGATAGACCGGGAAAGAGC | 555 | 62 |
14 | 3’-untranslated region 3 | AGCCCAGCAGCTCATCAC | TTCTCAGTGGGAGGGCTTC | 573 | 62 |
15 | 3’-untranslated region 4 | CAGCTCTGACTGGTGATGGA | AACGGGAAGTGGAGGAGAAT | 244 | 58 |