Exons

Sequence

PCR product

Annealing temperature

2

F: 5’ GATCCTGTATGGAGTGTCTGGC 3’

R: 5’GCTGAACTGCAGAGGACCTGGC3’

473 pb

65˚C

3

F: 5’ AGGCAAACAGTGGCTTTCTGGG 3’

R: 5’ ATGGGGCTCAAGTGGGGTAGGG 3’

415 pb

65˚C

4

F: 5’ CTGGTGTGACCCCATTTCTGCC 3’

R: 5’ CTTGGAGATACCAACACAAGCC3’

518 pb

65˚C

5

F: 5’ CAGATGTCCATGCATCCTTCCC 3’

R: 5’ CTGTCCTCACAGGGAAGGTCCC3’

462 pb

65˚C

6

F: 5’ ATCCCCAGAACGTAGGATGCCC3’

R: 5’ CAGCGTCCAGAACACTGAGCCC3’

421 pb

65˚C

7

F: 5’ AGCTCCTTTCTTAGCTTGGCCC 3’

R: 5’ CTTTGTTAGGCGCGAACCTCCC 3’

372 pb

65˚C

8-1

F: 5’ TGGTGATGGGAAAACGCAAGGG 3’

R: 5’ ACATGAAGCACACCAGGTAGGG 3’

884 pb

65˚C

8-2

F: 5’ CCCATGCAGAGCCCTACACGCG 3’

R: 5’ GCTTGGCCAGCAGCTTAAGGCG 3’

942 pb

66˚C

8-3

F: 5’ CCTGGAAGGAGACCAACATGGC 3’

R: 5’ TCAGGCTCAGGGTGGAGATGGC 3’

887 pb

68˚C

8-4

F: 5’ TTTTCTTCCCATTCCTGTCGGC 3’

R: 5’ TTCATTCAACAGGAGTGCAGGC 3’

911 pb

65˚C