Exons | Sequence | PCR product | Annealing temperature |
2 | F: 5’ GATCCTGTATGGAGTGTCTGGC 3’ R: 5’GCTGAACTGCAGAGGACCTGGC3’ | 473 pb | 65˚C |
3 | F: 5’ AGGCAAACAGTGGCTTTCTGGG 3’ R: 5’ ATGGGGCTCAAGTGGGGTAGGG 3’ | 415 pb | 65˚C |
4 | F: 5’ CTGGTGTGACCCCATTTCTGCC 3’ R: 5’ CTTGGAGATACCAACACAAGCC3’ | 518 pb | 65˚C |
5 | F: 5’ CAGATGTCCATGCATCCTTCCC 3’ R: 5’ CTGTCCTCACAGGGAAGGTCCC3’ | 462 pb | 65˚C |
6 | F: 5’ ATCCCCAGAACGTAGGATGCCC3’ R: 5’ CAGCGTCCAGAACACTGAGCCC3’ | 421 pb | 65˚C |
7 | F: 5’ AGCTCCTTTCTTAGCTTGGCCC 3’ R: 5’ CTTTGTTAGGCGCGAACCTCCC 3’ | 372 pb | 65˚C |
8-1 | F: 5’ TGGTGATGGGAAAACGCAAGGG 3’ R: 5’ ACATGAAGCACACCAGGTAGGG 3’ | 884 pb | 65˚C |
8-2 | F: 5’ CCCATGCAGAGCCCTACACGCG 3’ R: 5’ GCTTGGCCAGCAGCTTAAGGCG 3’ | 942 pb | 66˚C |
8-3 | F: 5’ CCTGGAAGGAGACCAACATGGC 3’ R: 5’ TCAGGCTCAGGGTGGAGATGGC 3’ | 887 pb | 68˚C |
8-4 | F: 5’ TTTTCTTCCCATTCCTGTCGGC 3’ R: 5’ TTCATTCAACAGGAGTGCAGGC 3’ | 911 pb | 65˚C |